Proc natl acad sci usa vol 95, pp 6222-6227, may 1998 evolution characterization of mads homeotic genes in the fern ceratopteris richardii. Ceratopteris brongn - antlerfern species: ceratopteris richardii brongn - triangle waterfern subordinate taxa this plant has no children. If your organization uses openathens, you can log in using your openathens username and password to check if your institution is supported, please see this list contact your library for more details.
Ceratopteris richardii is a fern species belonging to the genus ceratopteris, one of only two genera of the ceratopteridoideae subfamily of the pteridaceae it is one of several genera of ferns adapted to an aquatic existence. The wild-type spores of ceratopteris richardii were illuminated for 45 h under white light, to trigger the germination response, then cultured in the dark (closed circles), in. Here, we report a transgenic analysis in the fern ceratopteris richardii that reveals a role for leafy in maintaining cell divisions in the apical stem cells of both haploid and diploid phases of the lifecycle.
Ceratopteris richardii is quite similar to c cornuta and can only safely be discerned by close examination of the spores this species is used as model organism in plant genetics this species is used as model organism in plant genetics. 1 ceratopteris brongniart, bull sci soc philom paris sér 3, 8: 186 1821 antler ferns, water ferns, floating ferns [greek cerato, horned, and pteris, fern. Biological factors involved in reproductive barriers between two divergent races of ceratopteris richardii were investigated we used a combination of spore germination rates, qtl analysis of spore germination rates, and transmission ratio distortion (trd) of 729 rflps, aflps, and isozyme markers distributed across the genome on the basis of hybrid populations of 488 doubled haploid lines. Ceratopteris the genus ceratopteris is in the family pteridaceae in the major group pteridophytes (ferns and fern allies) statistics are at the end of the page. This comprehensive study of early embryology in ceratopteris richardii combines light microscopy with the first ultrastructural evaluation of any pteridophyte embryo emphasis is placed on ontogeny of the foot and placental transfer cells.
Germinating ceratopteris richardii spores have proven to be a useful single-cell system for analyzing light and gravity regulation of development (cooke et al, 1995 banks, 1999 chatterjee and roux, 2000 murata and sugai, 2000), but the lack of a gene sequence database and of a reliable way to suppress the expression of specific genes in. Ceratopteris richardii gametophytes and sporophytes are grown in tissue culture on c-fern nutrient 1% agar medium, ph 60 this media can be bought commercially or prepared from stock solutions as described in [ 11 ] (additional file 1 . Isolation and analysis of ceratopteris richardii antheridiogen c 1 isolation and analysis of ceratopteris richardii antheridiogen c adham alifarag submitted to the committee on undergraduate honors of baruch honors college. Regeneration in the fern ceratopteris richardii the demonstration of crosstalk between sugar signaling and the hor- mone ethylene signaling in angiosperms prompted us to test the effects of this hormone in combination with sugar on. Both processes can be induced in the laboratory in the homosporous fern ceratopteris richardii simply by altering the level of sugar supplement in the growth media, and in the case of apogamy, by also preventing fertilization [5.
The gametophyte generation of ceratopteris richardii is being used in a number of laboratories as a model plant system for developmental studies (chasan, 1992) the influence of gravity and light on developmental polarity of single cells of ceratopteris richardii gametophytes. First, i successfully induced apogamy from the fern ceratopteris richardii and discovered that the gametophytes begin to become committed to apogamy after 10 days of culture on inductive medium i then created a cdna library that represents genes with enhanced expression during commitment. A third species, ceratopteris richardii, can only be discerned from c thalictroides and c cornuta by the number of spores per sporangium moreover, ceratopteris species are known to hybridise c thalictroides is a very fast growing fern and is usually cultivated free-floating on the water surface, like all other ceratopteris species.
Figure 1 the lifecycle of ceratopteris richardii and its relation to microparticle-mediated transformation the c richardii lifecycle comprises two separate, free-living stages, the haploid gametophyte ( a - d ) and the diploid sporophyte ( e - g . Ceratopteris richardii is an aquatic fern grown in tropical and subtropical regions of the world it is proven to be a productive model system for studies in the genetics, biochemistry, and cell. Presents laboratory activities focusing on the development of sexually mature gametophytes from single-celled spores includes techniques for culture and manipulation of gametophyte development from spores that are applicable for hands-on activities for students at all levels (mkr. C-fern is a specially derived cultivar of a tropical fern, ceratopteris richardii over a three-week period, c-fern develops from a single-celled spore into a complex vascular plant with leaves and roots.
Expression in ceratopteris richardii gametophytes michael t ganger, julia a girouard, hannah m smith, beth a bahny, and sarah j ewing abstract: the development of male ceratopteris richardii brongn gametophytes (induction) is well studied. Introduction haploid gametophytes of many homosporous ferns, including ceratopteris richardii, develop as males or hermaphrodites the choice between these alternative developmental fates is. This page was last edited on 21 october 2017, at 04:26 text is available under the creative commons attribution-sharealike license additional terms may applyby.